Free minicircles of kinetoplast DNA in Crithidia fasciculata.
نویسندگان
چکیده
منابع مشابه
Structure of discontinuities in kinetoplast DNA-associated minicircles during S phase in Crithidia fasciculata
Kinetoplast DNA (kDNA) is a novel form of mitochondrial DNA consisting of thousands of interlocked minicircles and 20-30 maxicircles. The minicircles replicate free of the kDNA network but nicks and gaps in the newly synthesized strands remain at the time of reattachment to the kDNA network. We show here that the steady-state population of replicated, network-associated minicircles only becomes...
متن کاملIsolation and characterization of kinetoplast DNA networks and minicircles from Crithidia fasciculata.
7. Cook, J. R. 1968. The cultivation and growth of Euglena, in Buetow, D. E., ed., The Biology of Euglena, Academic Press, New York 1, 243-314. 8. Cooper, T. G. & Beevers, H. 1969. P-oxidation in glyoxysomes from castor bean endosperm. J . Biol. Chem. 244, 3514-20. 9. Cramer, M. & Myers, J. 1952. Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384-402. 10. Da...
متن کاملReplication of kinetoplast DNA in isolated kinetoplasts from Crithidia fasciculata. Identification of minicircle DNA replication intermediates.
The kinetoplast DNA (kDNA) of trypanosomes is comprised of thousands of DNA minicircles and 20-50 maxicircles catenated into a single network. We show that kinetoplasts isolated from the trypanosomatid species Crithidia fasciculata incorporate labeled nucleotides and support minicircle DNA replication in a manner which mimics two characteristics of minicircle replication in vivo: 1) the minicir...
متن کاملChanges in Organization of Crithidia fasciculata Kinetoplast DNA Replication Proteins during the Cell Cycle
Kinetoplast DNA (kDNA), the mitochondrial DNA in kinetoplastids, is a network containing several thousand topologically interlocked minicircles. We investigated cell cycle-dependent changes in the localization of kDNA replication enzymes by combining immunofluorescence with either hydroxyurea synchronization or incorporation of fluorescein-dUTP into the endogenous gaps of newly replicated minic...
متن کاملA single-stranded DNA-binding protein from Crithidia fasciculata recognizes the nucleotide sequence at the origin of replication of kinetoplast DNA minicircles.
A sequence-specific single-stranded DNA-binding protein from the trypanosomatid protozoan Crithidia fasciculata binds to a sequence of 12 nucleotides located at the origin of replication of kinetoplast DNA minicircles. This sequence, termed the universal minicircle sequence (UMS), is conserved in the kinetoplast DNA minicircles among species of the family Trypanosomatidae. The purified protein ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30096-0